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biotinylated goat antirabbit igg seconder antibody  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated goat antirabbit igg seconder antibody
    Biotinylated Goat Antirabbit Igg Seconder Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 19646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+goat+antirabbit+second+antibody/Biotinylated+Goat+Anti-Rabbit+IgG+Antibody/pm37798599-105-11-18
    Average 96 stars, based on 19646 article reviews
    biotinylated goat antirabbit igg seconder antibody - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: Characterization of a nuclear deformed epidermal autoregulatory factor-1 (DEAF-1)-related (NUDR) transcriptional regulator protein.
    Article Snippet: The cells were rinsed with PBS, fixed for 15 min in 4% paraformaldehyde, rinsed with PBS, permeablized for 7 min in 100% methanol at 220 C, rinsed with PBS, and then incubated at 25 C in normal goat serum (Vector Laboratories) for 20 min. .. The normal goat serum was removed and the slides were incubated with 1:1000 dilution of either preimmune rabbit serum or anti-NUDR serum overnight at 4 C. The slides were rinsed with PBS, incubated for 1 h at 25 C with either a 1:100 dilution of goat antirabbit antibody conjugated to fluorescein isothiocyanate (Sigma, St. Louis, MO) or a biotinylated goat antirabbit second antibody and fluoresceinconjugated avidin, and rinsed with PBS, after which coverslips were mounted on the slides using VECTASHIELD Mounting Medium (Vector Laboratories). .. For endogenous NUDR, indirect immunofluorescence was observed using fluorescein filters with an Olympus IMT-2 microscope.

    Avidin-Biotin Assay:

    Article Title: Characterization of a nuclear deformed epidermal autoregulatory factor-1 (DEAF-1)-related (NUDR) transcriptional regulator protein.
    Article Snippet: The cells were rinsed with PBS, fixed for 15 min in 4% paraformaldehyde, rinsed with PBS, permeablized for 7 min in 100% methanol at 220 C, rinsed with PBS, and then incubated at 25 C in normal goat serum (Vector Laboratories) for 20 min. .. The normal goat serum was removed and the slides were incubated with 1:1000 dilution of either preimmune rabbit serum or anti-NUDR serum overnight at 4 C. The slides were rinsed with PBS, incubated for 1 h at 25 C with either a 1:100 dilution of goat antirabbit antibody conjugated to fluorescein isothiocyanate (Sigma, St. Louis, MO) or a biotinylated goat antirabbit second antibody and fluoresceinconjugated avidin, and rinsed with PBS, after which coverslips were mounted on the slides using VECTASHIELD Mounting Medium (Vector Laboratories). .. For endogenous NUDR, indirect immunofluorescence was observed using fluorescein filters with an Olympus IMT-2 microscope.



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